Cellule staminali umane da liquido amniotico possono integrarsi e differenziarsi in linee epiteliali di polmone

Estratto da: Stem Cells

Human Amniotic Fluid Stem Cells Can Integrate and Differentiate Into Epithelial Lung Lineages.

Carraro G, Perin L, Sedrakyan S, Giuliani S, Tiozzo C, Lee J, Driscoll B, Turcatel G, Delanghe SP, Bellusci S, Minoo P, Atala T, De Filippo RE, Warburton D.

Developmental Biology, Regenerative Medicine and Surgery Program, Saban Research Institute, Childrens Hospital Los Angeles, Keck School of Medicine and School of Dentistry, 4661 Sunset Boulevard, Los Angeles, CA 90027, USA.
A new source of stem cells has recently been isolated from amniotic fluid (AFSC) with significant potential for regenerative medicine. These cells are multipotent, showing the ability to differentiate into cell types from each embryonic germ layer. We investigated the ability of human AFSC (hAFSC) to integrate into murine lung and to differentiate into pulmonary lineages after injury. Using microinjection into cultured mouse embryonic lungs, hAFSC can integrate into the epithelium and express the early human differentiation marker thyroid transcription factor 1 (TTF1). In adult nude mice, following hyperoxia injury tail vein injected hAFSC localized in the distal lung and expressed both TTF1 and the type II pneumocyte marker surfactant protein C (SPC). Specific damage of Clara cells through naphthalene injury produced integration and differentiation of hAFSC at the bronchioalveolar and bronchial positions with expression of the specific Clara cell 10-kD protein (CC10). These results illustrate the plasticity of hAFSC to respond distinctly to different types of lung damage by expressing specific alveolar versus bronchiolar epithelial cell lineage markers depending on the type of injury to recipient lung.